Progress in the field of microbial hydrogen production and metabolism in the Yantai coastal zone

Hydrogen is a kind of clean energy, it is the power fuel of the next generation of new energy vehicles, and it has extremely broad application prospects. Microbial hydrogen production is one of the important production methods, which belongs to the emerging development field of green manufacturing and is used to support the sustainable development of economy and society.

The "Electrical Microbiology" research team of the Yantai Coastal Zone Research Institute of the Chinese Academy of Sciences, through heat treatment coupled with the addition of Nano-ferrihydrite, directed enrichment from the samples of the Yellow River Delta coastal wetlands, Swan Lake and Bohai Sea sediments, etc. A number of fermentative dissimilating iron-reducing bacteria strains with electrochemical activity have been isolated and have been authorized by invention patents. The study found that the process of reduction of nanometer ferrihydrite significantly changed the microbial community structure and its metabolic pathways, while improving carbon metabolism and hydrogen production efficiency (as shown in Figure 1).

In addition, the study takes hydrogen-producing strains as the research object, and through transcriptomics, electrochemistry, enzymology, and stoichiometric analysis, reveals the mechanism by which the dissimilated iron reduction process promotes the enrichment of hydrogen-producing strains and improves carbon metabolism and hydrogen production efficiency . The study found that the iron reduction process in the nanometer ferrihydrite is coupled with the fermentation process, making the hydrogen production reaction relatively easier to occur thermodynamically; the iron reduction process will accept electrons, thereby changing the direction of carbon flow and electron flow, resulting in more More hydrogen; nanometer ferrihydrite can also regulate the gene expression of the strain. Genes such as the two-component system (TCS), glucose phosphate transfer system (PTS), NADH dehydrogenase and hydrogenase are all up-regulated to varying degrees; in addition, The iron reduction process can also consume protons, alleviate the accumulation of organic acids during fermentation, and maintain a relatively stable pH value, which is more conducive to hydrogen production (see Figure 2).

The series of studies established technical methods for screening high-hydrogen-producing strains, and obtained series strain resources. The research results will help promote technological upgrading in the field of microbial hydrogen production and provide technical support for green manufacturing and sustainable development of the coastal zone economy. Related research results have been published in domestic and foreign journals such as ACS Sustainable Chemistry & Engineering, Fuel, Bioresource Technology and Science China Technological Sciences.

The research was supported by the Strategic Leading Science and Technology Project of the Chinese Academy of Sciences (Class A) (XDA22050301), the National Natural Science Foundation of China's major research plan for the development of the mechanism of the hydrosphere microorganisms driving the circulation of the earth's elements (91751112), and the "Taishan Scholar" Young Expert Program ( tsqn20161054) and other funding.


Figure 1. Reduction and directed enrichment of Clostridium hydrogen-producing Clostridium by ferrihydrite


Figure 2. Reduction of ferrihydrite promotes Clostridium pasteurianum's hydrogen production pathway

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